glpK [2017-04-24 15:34:12]

glycerol kinase

Locus: BSU09290

Isoelectric point: 4.99

Molecular weight: 54.91 kDa

Protein length: 496 aa

Gene length: 1488 bp

Function: glycerol utilization

Product: glycerol kinase

Essential: no

E.C.: 2.7.1.30

Synonyms

Genomic Context

Loading

Categories containing this gene/protein

• Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of glycerol/ glycerol-3-phosphate
• Groups of genes → Phosphoproteins → Phosphorylation on a His residue

This gene is a member of the following regulons

• AbrB regulon, CcpA regulon, GlpP regulon

Gene

Coordinates: 1,003,344 → 1,004,834

The protein

Catalyzed reaction/ biological activity

• ATP + glycerol = ADP + sn-glycerol 3-phosphate (according to Swiss-Prot)

Protein family

• FGGY kinase family (according to Swiss-Prot)

Structure

• 3H46 (from Enterococcus casseliflavus, complex with glycerol) PubMed

Expression and Regulation

Operons

• Genes: glpF-glpK
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • CcpA: repression, PubMed, in CcpA regulon
  • GlpP: antitermination, /antitermination via GlpP-dependent RNA switch, in GlpP regulon
  • AbrB: activation, PubMed, in AbrB regulon
  • ResD: repression, PubMed, in ResD regulon

  Regulation

  • induction by glycerol (GlpP) PubMed
  • repressed under anaerobic conditions (ResD) PubMed

Biological materials

Mutant

• BKE09290 (glpK::ermC) (available at the BGSC and in Jörg Stülke's lab)
• GP1865 (glpK::ermC) (available in Jörg Stülke's lab)

Labs working on this gene/protein

• Josef Deutscher, Paris-Grignon, France

References

Loading